Comparison Analysis of Species Composition of Entomopathogenic Fungi Isolated from Bark Beetles (Coleoptera: Scolytidae)

Abstract

The monitoring outbreaks of bark beetles, flight activity of imago and development of larvae were conducted with pheromone traps and trap logs. We found more than 50 dead adults with symptoms white muscardine from several points under the bark. Healthy adults of Ips typographus without any pathogenic symptoms collected from pheromone traps and trap logs under bark in the forest Czech Bohemian Switzerland (České Švýcarsko). For adaptation bark beetles Ips typographus in the laboratory conditions and excluded any other abiotic factors as a potential reason for their mortality. They were placed in bark in the Petri plates with filter paper and treated by 1 ml of entomopathogenic fungi conidial suspensions.The isolates Bcz38 and Inc2 showed low level of mortality of Ips typographus. But the isolate Bcz2, Bcz8, Bcz9, Bcz12-Bcz25 of B.bassiana and Bcz31 B.pseudobassiana had high virulence against of Ips typographus 100%.