Human Adipose Stem Cells in Collagen on Angiogenesis Process of Burn Healing in Rat Model: Its Number of Blood Vessels

Abstract

Angiogenesis in the burn wound healing process is important. The application stem cell therapy in burn wound problem is expected to increase formation of new blood vessel so that the distribution of oxygen and nutrients working well. The purpose of this study was to known effect of human Adipose Derived Stem Cell (hADSC) in collagen gel on the average number of blood vessels based on time dependent. This study used 25 male rats Sprague Dawley who had received three internal wounds with different treatments. The treatments used hADSC in collagen gel, collagen gel only and without treatment. The rats were divided into 5 groups based on the duration of observation day 0, 7, 14, 21 and 28. Rat without burn wound were used as control. Observation blood vessels number using hematoxylin-eosin staining and calculated of average number blood vessels from five filed in five mice. Statistical analysis used ANOVA one way with significance value p<0.05. The average number blood vessels increase significantly until day 21 and decreased on day 28, both in untreated burn wound, collagen gel and hADSC in collagen gel. The treatment with hADSC in collagen gel was not significantly different from the collagen gel group or without treatment at day 7, 14 and 21. Even though the average of blood vessels of treatment with hADSC in collagen gel was not significantly different but our research had showed that treatment with hADSC in collagen gel was higher number of blood vessels than another group. Decrease of blood vessel number at 28 days indicated start of near homeostasis state and treatment hADSC in collagen gel has significantly decreased than another group. Our research showed that angiogenesis was time dependent and hADSC in collagen gel have not a role in increase of number blood vessels but we have indicated that hADSC might be have a role in qualitative improvement of angiogenesis.