TY  - JOUR
AU  - Wang, Biaoshi 
AU  - Li, Wenfeng 
AU  - Liu, Shumin 
AU  - Hu, Xiaojun 
AU  - Zhang, Shiqi 
AU  - Yang, Shengyuan 
PY  - 2023
TI  - Extraction Process Optimization and Functional Characteristics of by Papain Solubilized Collagen from Large Yellow Croaker (Pseudosciaena crocea) Skin
JF  - American Journal of Biochemistry and Biotechnology
VL  - 19
IS  - 4
DO  - 10.3844/ajbbsp.2023.347.357
UR  - https://thescipub.com/abstract/ajbbsp.2023.347.357
AB  - Through collagen extraction from large yellow croaker (Pseudosciaena crocea) skin using an enzymatic method, the extraction optimization and characterization of collagen from raw materials after papain hydrolysis pretreatment are studied. The extraction process was optimized using the response surface methodology in this study. The findings show that the optimal conditions are a ratio (solvent to solid) of 25.0 mL/g, a time of 42.0 h, a temperature of 40.0°C and a pH of 7.0. Under these optimum conditions, the collagen extraction rate is 66.17%. Based on the FTIR spectra analysis, collagen can maintain a high-interacted structure, and collagen peptides have a complete triple helix structure. In addition, the SEM analysis shows that the extracted collagen has well-defined fibril morphologies. Also, the foaming capacity and emulsifying ability of extracted collagen are superior to commercial type I collagen.